Single step aqueous two-phase extraction for downstream processing of C-phycocyanin from Spirulina platensis
نویسندگان
چکیده
منابع مشابه
Purification of C-phycocyanin from Spirulina platensis in Aqueous Two-Phase Systems using an Experimental Design
C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the...
متن کاملExtraction and Purification of C-phycocyanin from Spirulina platensis in Conventional and Integrated Aqueous Two-Phase Systems
Extrato aquoso de C-ficocianina de Spirulina platensis, com e sem resíduos celulares, foi purificado em sistema aquoso bifásico (SAB) polietilenoglicol (PEG)/fosfato de potássio variando-se a massa molar do PEG, a pH 6, mais promissor do que a pH 7. Para os ensaios realizados com cada massa molar, com diferentes percentuais de PEG e sal, as concentrações (mg mL), as purezas (OD 620/OD 280) e os...
متن کاملC-PHYCOCYANIN EXTRACTION FROM Spirulina platensis WET BIOMASS
C-Phycocyanin is a natural blue dye used in food and pharmaceutical industry. In the present study, a simple and efficient method to extract C-phycocyanin from Spirulina platensis wet biomass is reported. The extractions were carried out using six different methods, including chemical (organic and inorganic acid treatment), physical (freezing and thawing, sonication, homogenization) and enzymat...
متن کاملApplication of an Ultrafine Shearing Method for the Extraction of C-Phycocyanin from Spirulina platensis.
Cell disruption is an important step during the extraction of C-phycocyanin from Spirulina platensis. An ultrafine shearing method is introduced and combined with soaking and ultrasonication to disrupt the cell walls of S. platensis efficiently and economically. Five kinds of cell disruption method, including soaking, ultrasonication, freezing-thawing, soaking-ultrafine shearing and soaking-ult...
متن کاملConformational Studies on C-Phycocyanin from Spirulina platensis
Bile Pigments, Protein Interaction, Conformation, Denaturation The chromophore-protein interactions of C-phycocyanin (C-PC) from Spirulina platensis have been studied by following the partial and complete denaturation with UV-Vis spectroscopy. From comparison with published MO calculations, an elongated conformation of the chromophore is sug gested for native C-PC, a cyclic one for denatured C...
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ژورنال
عنوان ژورنال: Journal of Food Science and Technology
سال: 2014
ISSN: 0022-1155,0975-8402
DOI: 10.1007/s13197-014-1287-9